Canada’s standardization of environmental DNA (eDNA) analysis reaches new heights with the launch of the second national eDNA standard, CSA W219. This groundbreaking standard, developed by the Canadian Standards Association (CSA), establishes performance criteria for targeted quantitative polymerase chain reaction (qPCR). CSA W219 is a companion to the first Canadian eDNA Standard: CSA W214 that addresses reporting, analytical performance, and field application criteria, setting the gold standard for reliability and precision in eDNA studies.
User-Focused Approach: Designed for testing entities, industrial proponents, regulatory agencies, environmental NGOs, and more, CSA W219 fosters collaboration and standardization. It’s a user-centric guide, ensuring competency across diverse sectors involved in eDNA studies.
Impact and Application: CSA W219 isn’t just a document; it’s a tool for effective project evaluation, enhancing confidence in decision-making related to conservation and resource management. It sets performance criteria for targeted qPCR assays, to be used alongside CSA W214 and other relevant standards.
Flexibility for the Future: Acknowledging the dynamic nature of scientific progress, CSA W219 remains adaptable to emerging technologies. While focusing on widely used eDNA detection methods, it leaves room for future developments, ensuring relevance in the evolving field of genetics research.